Approaches/skills and techniques:
- Bacterial and mammalian cell culture
- Protein purification
- Protein characterisation
- Identify protease cleavage sites
- Protease assays
- Peptide sequencing and functional analyses
- Western Blot
Through this project, prospective students will gain skills in numerous scientific techniques that are broadly applicable throughout the biomedical science disciplines. Based at the world-class Translational Research Institute, the students will access the state-of-the-art facilities and join a vibrant team of talented biomedical researchers within the Plasticity research group and broader Australian Prostate Cancer Research Centre – Queensland.
The overarching hypothesis of the project is that protein MNamp will play a functional role in modulating the aggressive characteristics of n-MYC in MYCN amplified NB and NEPC. The aims of the project are:
Aim 1: Express and purify novel protein MNamp;
Aim 2: Identify and characterise functional domains of protein MNamp;
Aim 3: Determine a functional role for protein MNamp via treatment of NB and NEPC cell lines in vitro;
Aim 4: Identify potential inhibitors of protein MNamp using in silico and in vitro small molecule library screens.
Required skills and experience
Potential students should have a keen interest in cancer biology and demonstrate a high level of organisation, attention to detail and ability to work in a team environment. Protein purification knowledge preferred but not required.
Please contact Dr Brett Hollier for details.