Dr Pawel Sadowski
Faculty of Science,
School of Biology & Environmental Science
Biography
Pawel completed his PhD at the University of Cambridge (Cambridge, UK) having developed quantitative proteomics methods to study membrane protein translocation under the guidance of Prof Kathryn Lilley. He then undertook postdoctoral research in the area of quantitative proteomics in laboratories of Prof Thomas Neubert at the New York University (New York, USA) and Prof Robert Yang at the University of New South Wales (Sydney, AU) and joined QUT in 2013 to lead CARF Proteomics laboratory. Pawel's role is to supervise research that relies on Liquid Chromatography Mass Spectrometry (LCMS) and Gas Chromatography Mass Spectrometry (GCMS) instrumentation and provide strategic direction around decisions affecting the Proteomics laboratory and its user-base. In collaboration with UQ SVS and CARF Genomics laboratory, he is automating sample preparation using robotics and developing pipelines that leverage modern mass spectrometry data acquisition approaches (SWATH), deep learning algorithms and long-read RNA sequencing (Iso-Seq) to enable quantitative proteomics and discovery of credible biomarkers implicated in animal health, behavior and enviornmental adaptation.Personal details
Positions
- Research Fellow (Proteomics)
Faculty of Science,
School of Biology & Environmental Science
Keywords
quantitative mass spectrometry, proteomics, data-independent acquisition (SWATH), multiple reaction monitoring (MRM), multi-omics
Research field
Biochemistry and cell biology, Medical biochemistry and metabolomics
Field of Research code, Australian and New Zealand Standard Research Classification (ANZSRC), 2020
Qualifications
- Ph D in Biochemistry (University of Cambridge)
Publications
- Paramasivan, S., Ashick, M., Dudley, K., Satake, N., Mills, P., Sadowski, P. & Nagaraj, S. (2024). VPBrowse: Genome-based representation of MS/MS spectra to quantify 10,000 bovine proteins. Proteomics, 24(14), Article 2300431. https://eprints.qut.edu.au/247948
- Paramasivan, S., Morrison, J., Lock, M., Darby, J., Barrero, R., Mills, P. & Sadowski, P. (2023). Automated Proteomics Workflows for High-Throughput Library Generation and Biomarker Detection Using Data-Independent Acquisition. Journal of Proteome Research, 22(6), 2018–2029. https://eprints.qut.edu.au/240781
- Navone, L., Moffitt, K., Behrendorff, J., Sadowski, P., Hartley, C. & Speight, R. (2023). Biosensor-guided rapid screening for improved recombinant protein secretion in Pichia pastoris. Microbial Cell Factories, 22(1), Article 92. https://eprints.qut.edu.au/240118
- Turner, N., Abeysinghe, P., Sadowski, P. & Mitchell, M. (2023). Omics Analysis of Extracellular Vesicles Recovered from Infant Formula Products and Milk: Towards Personalized Infant Nutrition. Molecular Nutrition and Food Research, 67(19), Article 2300404. https://eprints.qut.edu.au/242291
- Bryan, E., Trim, L., Sadowski, P., Paramasivan, S., Kim, J., Gough, K., Worley, S., Maidment, T., Carey, A., Mihalas, B., McLaughlin, E. & Beagley, K. (2023). Prophylactic and therapeutic vaccination protects sperm health from Chlamydia muridarum-induced abnormalities. Biology of Reproduction, 108(5), 758–777. https://eprints.qut.edu.au/241668
- Turner, N., Abeysinghe, P., Flay, H., Meier, S., Sadowski, P. & Mitchell, M. (2023). SWATH-MS Analysis of Blood Plasma and Circulating Small Extracellular Vesicles Enables Detection of Putative Protein Biomarkers of Fertility in Young and Aged Dairy Cows. Journal of Proteome Research, 22(11), 3580–3595. https://eprints.qut.edu.au/243863
- Turner, N., Abeysinghe, P., Kwan Cheung, K., Vaswani, K., Logan, J., Sadowski, P. & Mitchell, M. (2022). A Comparison of Blood Plasma Small Extracellular Vesicle Enrichment Strategies for Proteomic Analysis. Proteomes, 10(2), Article 19. https://eprints.qut.edu.au/236855
- Ravuri, H., Noor, Z., Mills, P., Satake, N. & Sadowski, P. (2022). Data-Independent Acquisition Enables Robust Quantification of 400 Proteins in Non-Depleted Canine Plasma. Proteomes, 10(1), Article 9. https://eprints.qut.edu.au/241669
- Ghodasara, P., Satake, N., Sadowski, P., Kopp, S. & Mills, P. (2022). Investigation of cattle plasma proteome in response to pain and inflammation using next generation proteomics technique, SWATH-MS. Molecular Omics, 18(2), 133–142. https://eprints.qut.edu.au/232191
- Noor, Z., Paramasivan, S., Ghodasara, P., Chemonges, S., Gupta, R., Kopp, S., Mills, P., Ranganathan, S., Satake, N. & Sadowski, P. (2022). Leveraging homologies for cross-species plasma proteomics in ungulates using data-independent acquisition. Journal of Proteomics, 250, Article 104384. https://eprints.qut.edu.au/234700
QUT ePrints
For more publications by Pawel, explore their research in QUT ePrints (our digital repository).
Filter publications:
A complete list of publications is available at: https://www.qut.edu.au/about/our-people/academic-profiles/pawel.sadowski
Supervision
Current supervisions
- Application of third-generation proteomics to study protein isoform dynamics during insect development
PhD, Associate Supervisor
Other supervisors: Dr Kevin Dudley - Genome to Phenome: Multi-Omics and Epigenetic Mechanisms Shaping Behavioural Phenotype
PhD, Principal Supervisor
Other supervisors: Dr Kevin Dudley - Uncovering Isoform Dynamics and Leveraging Long-Read Sequencing to Refine Genome Annotation of Helicoverpa armigera
PhD, Associate Supervisor
Other supervisors: Dr Kevin Dudley
Completed supervisions (Doctorate)
- Bioprospecting, and Characterisation of plastic and plasticiser degrading enzymes from Great Australian Artesian Basin thermophiles for biorecycling and bioremediation (2024)
- The development and application of an analytical pipeline for extracellular vesicle-based diagnostics (2024)
- Characterisation of the Australian Great Artesian Basin's Caldaquabacillus species and potential role in thermophilic plastic biodegradation (2023)
- Improving SWATH-MS data analysis using multi-omics approach (2022)
Completed supervisions (Masters by Research)
Supervision topics
The supervisions listed above are only a selection.