Yarrowia lipolytica is a yeast that is capable of using a host of lipids as a carbon source. The yeast is used for equine and pet food supplements and holds great promise a probiotic and nutritional supplement.
We have six unique Australian strains that need to be characterised and compared using various omics platforms. Additionally, growth in various lipid-rich aqueous wastewaters will be compared. Wild type strains will also be genetically altered to develop strains with improved growth and substrate utilisation efficiencies.
While this is of a fundamental nature, this research aims to develop a fermentation process that generates Yarrowia biomass on a commercial scale.
As part of this research project, you'll be involved in:
- high-throughput assays
- fatty acid compositional analyses and pathway mapping
- submerged fermentation in shake flasks (250ml) and bioreactors (500ml to 1000 litres)
- adaptive laboratory evolution using radiation, chemical and advanced plasma technologies.
We aim to generate fundamental knowledge about a yeast that has immense potential as an industrial microbe. This knowledge will be used on lipid substrates to generate high-value microbial biomass.
Skills and experience
To be considered for this research project, you should have demonstrable skills and/or experience with:
- working in a laboratory
You may be able to apply for a research scholarship in our annual scholarship round.
Contact the supervisor for more information.