Institute of Health and
Biomedical Innovation
a university for the real world

Equipment

Microscopes

Microscopes

The Cell Analysis Facility at IHBI provides access to a wide range of microscope platforms including both routine and advanced optical and electron microscopes. The technology capabilities include scanning electron microscopy, bright field microscopy (phase contrast and DIC), multi channel fluorescence microscopy, confocal laser scanning microscopy, and is fully equipped for live-cell imaging.

InstrumentCapabilities and features
Leica TCS SP5 Confocal Microscope
  • Laser scanning confocal microscope
  • DMI6000 inverted stand
  • High resolution: 'optical slicing' of samples, Z-stack
  • Laser excitation: 8 laser lines (405, Multiline Argon laser [458, 475, 488, 496, 514], 561, 633 nm)
  • 4 tunable spectral detectors (PMTs) and 1 transmitted light detector
  • High resolution objectives
  • Fast galvo stage and high speed 8kHz resonant scanner available
  • Environmental chamber for the control of temperature, CO2 concentration, and humidity guarantees ideal conditions for prolonged time-lapse imaging
  • Imaging diverse sample formats from cultured cells on 2D and 3D structures
Leica AF6000LX Widefield Microscope
  • Fluorescence imaging
  • DMI6000 inverted stand
  • Long working distance 10x, 20x and 40x dry, and 63x glycerol objectives
  • Differential Interference Contrast (DIC)
  • External filter wheel for fast multi-colour acquisition
  • X-Y-Z motorised stage adaptable to a variety of sample formats including multi-well and other cell culture plates and microscopy slides
  • Tiling of large samples with excellent stitching of image sets.
  • Live-cell time lapse imaging in DIC and Fluorescence of multiple sites in parallel
  • Environmental chamber for the control of temperature, CO2 concentration, and humidity guarantees ideal conditions for prolonged time-lapse imaging
Nikon Eclipse TiS Inverted Microscope with Fluorescence
  • Epi-fluorescence imaging: DAPI, FITC, Cy3 and Cy5 filter cubes
  • Transmitted light - Phase Contrast
  • NIS-Br imaging software
Nikon Eclipse Ts2 Routine Inverted Microscope
  • LED illumination for diascopic and epi-fluorescence imaging
  • DAPI, FITC and TRITC filter cubes
  • Phase Contrast
Hitachi TM3000 ESEM
  • Benchtop Environmental Scanning microscope: no sample coating required
  • Backscattered imaging at 5 or 15kV beam energy
  • Charge-up reduction mode for insulating samples
  • Tilt-and-Rotate and Cool stage

Flow cytometry

Flow cytometry

The flow cytometry resources provide multi-parameter flow cytometric analysis for the evaluation of cell proliferation and development, apoptosis, presence of internal and external cell markers and proteins of interest, cell cycle analysis and much more.

InstrumentCapabilities and features
BD FACSCelesta Flow Cytometer
  • High-speed, multi-colour, digital analyzer
  • 3 lasers (405, 488 and 561nm)
  • 12 detectors
  • Software: FACSDiva - allows for automatic compensation setup and allows for compensation after data acquisition. This minimizes the amount of sample needed to obtain correct compensation before data acquisition.

Analysis software

Analysis software

InstrumentCapabilities and features
MetaMorph
  • Offline image analysis and rendering
  • Integrated Morphometry Analysis allows objects to be measured and separated into user-definable classes
  • Analyse cell signaling, cell counting, protein expression and more
  • Automate complex, multi-parametric experimental protocols
FloJo
  • Flexible and automatable analysis software for flow cytometry data