Overview
Topic status: We're looking for students to study this topic.
Overview
Wound healing is a complex process which involves interaction between cell surface molecules, the extracellular matrix and regulation of cell signalling and cell migration. Mice lacking key cell surface molecules provide ideal models to investigate wound healing.
TMEM26 is a cell surface molecule that has been implicated in retarded cell migration and disrupted cell polarity. We propose to utilise the TMEM26 knock–out mice to investigate the effect of this cell surface molecule on wound healing and keratinocyte cell migration. This research may provide new insights into the key regulators of cell migration and possible clinical targets for wound healing.
Hypothesis
TMEM26 has a key role in keratinocyte migration and we hypothesise that further investigation of TMEM26 will provide vital insights into the molecular pathways involved in wound healing.
Aims
- Examine the role of TMEM26 in wound healing in–vivo
- Determine if TMEM26 –/– keratinocytes have altered cell migration in–vitro.
- Determine if the TMEM 26 –/– keratinocytes have disrupted cell signalling pathways.
Methods and techniques
- Cell culture (mouse keratinocytes)
- Cell migration assays
- Western blot (analysis of protein expression levels)
- Mouse wound healing assays (this animal work can be undertaken if desired but is not necessary to complete the project)
- Immunohistochemistry
- Study level
- PhD, Masters, Honours
- Supervisors
- QUT
- Organisational unit
Science and Engineering Faculty
- Research area
- Keywords
- enhanced wound healing , wound healing , wound, TMEM26, knock–out mice
- Contact
- For more information, please contact one of the supervisors:
Dr Kerry Manton
Professor Zee Upton
