Overview
Topic status: We're looking for students to study this topic.
Both the Insulin like growth factor (IGF) and Transforming growth factor–beta (TGF–β) families of proteins are potent stimulators of cell function during development and tissue repair processes. TGF–β1 is produced in a latent form and needs to be activated in order to exert its cellular effects. An important and physiologically relevant method of activation involves protein complex formation on the cell surface with the cation–independent mannose 6–phosphate/insulin–like growth factor–II receptor (CIMPR).
On the cell surface, the CIMPR complexes with other cellular components, including urokinase plasminogen activator (uPA) and its receptor (uPAR), plasminogen and latent TGF–β1. The association drives the conversion of plasminogen to the active serine protease plasmin, which then cleaves and activates latent TGF–β1. Furthermore, it known that TGF–β1 mediated signalling within the cell can be modulated in the presence of IGF signal cascade activation.
We are particularly interested in the interaction of IGF–II with the CIMPR and the possible role of this interaction on TGF–β1 modulated cellular effects. This research is novel in that it challenges the current paradigm surrounding the purpose of the IGF–II, CIMPR interaction and will reveal possible connections between the IGF and TGF–β systems.
Methods and techniques developed
This project would suit someone who is interested in cell and molecular biology, especially as it applies to the fields of skin and bone tissue biology.
Techniques used include:
- cell culture
- western blotting
- real time Quantitative PCR
- immunocytochemistry
- Study level
- Honours
- Supervisors
- QUT
- Organisational unit
Science and Engineering Faculty
- Research area
- Contact
- Please contact a supervisor for enquiries.
Professor Zee Upton
Dr Tony Parker
Associate Professor David Leavesley
