Overview
Topic status: We're looking for students to study this topic.
Researchers in our team have recently developed a range of chimeric VN:IGF-I proteins that aim to re-produce the cellular effects of multiprotein VN:IGFBP:IGF-I complexes, a promising wound-healing agent, in a single entity. Similar to the multiprotein VN:IGFBP:IGF-I complex, the chimeric proteins influence cells through the activation of the v-integrin and the type-1 IGF (IGF-1R) cell surface receptors. The level of activation of each of these receptors is critical to the function of the chimeras.
Therefore, an understanding of the binding capabilities of the respective VN and IGF-I components is of critical importance. This project aims to further our understanding of the chimeric proteins through structural (binding) and functional experiments focussing on the IGF-I components of the chimeric proteins. The results from this study will influence future design and production of chimeric proteins that are ultimately aimed at providing an effective wound-healing therapy.
Hypothesis: That due to structural constraints, the VN:IGF-I chimeras differ in their presentation to cell surface receptors and therefore activate these receptor with varying affinities causing differing functional responses in cells.
Methods and techniques that will be developed in the course of this project:
- Recombinant protein expression
- Protein purification
- Cell based assays: Cell growth, migration and intracellular signalling
- Binding studies: BIACORE
- Study level
- Honours
- Supervisors
- QUT
- Organisational unit
Science and Engineering Faculty
- Research area
- Contact
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Please contact the supervisor.
Dr Derek Van Lonkhuyzen
Dr Gary Shooter
Professor Zee Upton
