Overview
Topic status: We're looking for students to study this topic.
Our research has developed a range of chimeric extracellular matrix (ECM):growth factor (GF) proteins with the aim of generating a cost effective wound healing therapeutic. These chimeras are directed at reproducing the beneficial effects of combinations of the individual components but in a form that is significantly more cost effective to produce, process and implement as a wound healing therapy. Interactions between proteins and receptors are complex and can be affected by a wide range of factors. Optimization of the peptide sequence linking the two protein domains together, as well as that between the protein and a purification tag, can greatly influence the function of the protein, for this reason there is a need to understand the properties of peptide linker sequences that conserve or even enhance the function of the incorporated protein species. Furthermore, there are a range of expression systems available for the production of recombinant proteins and each has its advantages and disadvantages. A comparative study is required to elucidate which system works best with regard to optimised linker sequences.
Hypothesis: That a range of linker sequences will generate a range of functional potencies within an ECM:GF chimera, therefore an optimal linker sequence may increase the potency of the chimeric protein.
Methods and techniques that will be developed in the course of this project:
- Computer modelling of candidate linker sequences
- Molecular generation of recombinant chimeric constructs
- Protein expression in yeast, insect and bacterial systems
- Cell based assays: Cell growth, migration and intracellular signalling to determine function of candidate linkers
- Structural determination of linker sequence effect on protein:receptor interaction through in vitro binding studies: BIACORE
- Study level
- Honours
- Supervisors
- QUT
- Organisational unit
Science and Engineering Faculty
- Research area
- Contact
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Please contact the supervisor.
Dr Derek Van Lonkhuyzen
Professor Zee Upton
