Overview
Topic status: We're looking for students to study this topic.
The ultimate goal of this project is to identify and profile the biomarkers that are expressed during the first days after a soft tissue injury in rats. Soft tissue injuries involving muscles and the neurovascular system often accompany bone fractures and represent one of the major complications for bone fracture healing. In order to improve the prevention, assessment and treatment of these traumatic injuries, understanding the interaction between soft tissue injuries and bone fractures is essential. The Trauma Research Group at IHBI is therefore establishing a small animal soft tissue injury model, which will provide a research platform to study soft tissue injuries alone and in combination with bone fractures in rats.
The subjective assessment and monitoring of closed soft tissue injuries represents a significant clinical challenge, and it is hoped that this research will ultimately lead to the characterisation of biomarkers that allow the non-invasive assessment of closed soft tissue injuries and the monitoring of the healing thereof.
The specific goal of this project is to optimise the analytical techniques required for the discovery and identification of biomarkers of soft tissue damage in rats with and without soft tissue injury.
This is a collaborative project which involves researchers from the Tissue Repair and Regeneration Program (IHBI) and the Trauma Research Group (IHBI).
Methods and techniques that will be developed in the course of this project:
This project will adopt cutting edge protein isolation, identification and quantitation techniques such as high pressure liquid chromatography (HPLC), liquid chromatography tandem mass spectrometry (LC-MS/MS), Surface Enhanced Laser Desorption/Ionisation Time Of Flight Mass Spectrometry (SELDI-TOF/MS), 2D-gel electrophoresis incl 2D-DIGE as well as more traditional protein biochemistry techniques such as SDS PAGE, immunological techniques and western blot analysis.
- Study level
- PhD, Honours
- Supervisors
- QUT
- Organisational unit
Science and Engineering Faculty
- Research area
- Contact
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Please contact the supervisor.
Dr Tony Parker
Dr Roland Steck
