Forensic collection and profiling of DNA from hard surfaces

Overview

Topic status: We're looking for students to study this topic.

Project Summary

The problem of obtaining a usable DNA profile from minute amounts of biological evidence collected from a dry hard surface at the crime scene is commonly encountered in forensic investigations.  The principal aim of this project is to develop the optimal operating parameters for this type of DNA collection and analysis using techniques that are commonly available to investigators and forensic laboratories.  To do this, we will grow cells in tissue culture flasks to various levels of confluence, and characterise the number of cells per unit area by microscopy.  The media will then be removed, and the flask will be allowed to dry completely to model trace cell debris.  The cells will be collected from the tissue culture plastic using standard forensic tape.  DNA will be isolated and purified from these samples using standard forensic techniques, and qPCR will be used to determine the efficiency with which forensic DNA loci can be amplified.  Correlating these data with the cell density will establish the lower limit of detection.

Expected outcomes, applications and/or benefits

This project addresses an issue that has been raised by the Queensland police.  Specifically, there has been no systematic study of this issue and no guidelines exist to aid investigators when collecting and analysing trace contact biological material deposited during a crime.  This project will establish the lower limit of detection for forensic DNA analysis this specific type of sample.  Ultimately, we will be able to provide the law enforcement community with guidelines for the reliable collection and analysis of these specific sample types.  Depending on the outcome of the project, these data may be publishable in a forensic journal.

Required student skills/experience

It is recommended that the students have completed LQB680 Forensic DNA Profiling prior to participating in this project.

Duration of project

10 weeks, starting from December 10th and finishing on February 15th
Number of hours per week expected: 20 Hrs per week.

Study level
Vacation research experience scholarship
Supervisors
QUT
Organisational unit

Science and Engineering Faculty

Research area

Cell and Molecular Biosciences

Keywords
forensic, DNA, biological, evidence
Contact
Contact the supervisor for more information